GAA Antibody / Glucosidase alpha acid

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NSJ Bioreagents
Product Code: NSJ-F54279
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54279-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54279-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the GAA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) HeLa, 2) MCF7, 3) SW620 and 4) A549 cell lysate with GAA antibody. Predicted molecular weight ~105 kDa.
2 / 5
Western blot testing of human 1) A549, 2) MCF7, 3) SW620 and 4) PC-3 cell lysate with GAA antibody. Predicted molecular weight ~105 kDa.
3 / 5
Western blot testing of human placenta lysate with GAA antibody. Predicted molecular weight ~105 kDa.
4 / 5
IHC testing of FFPE human placenta tissue with GAA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
IHC testing of FFPE human liver tissue with GAA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HeLa, 2) MCF7, 3) SW620 and 4) A549 cell lysate with GAA antibody. Predicted molecular weight ~105 kDa.
Western blot testing of human 1) A549, 2) MCF7, 3) SW620 and 4) PC-3 cell lysate with GAA antibody. Predicted molecular weight ~105 kDa.
Western blot testing of human placenta lysate with GAA antibody. Predicted molecular weight ~105 kDa.
IHC testing of FFPE human placenta tissue with GAA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with GAA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the GAA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 174-203 from the human protein were used as the immunogen for the GAA antibody.
Limitation:
This GAA antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P10253