HLA-B Antibody (MHC Class I)

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NSJ Bioreagents
Product Code: NSJ-F54291
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54291-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54291-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HLA-B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
IHC testing of FFPE human tonsil tissue with HLA-B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 6
IHC testing of FFPE human tonsil tissue with HLA-B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 6
IHC testing of FFPE human spleen tissue with HLA-B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 6
IHC testing of FFPE rat spleen tissue with HLA-B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 6
Western blot testing of human 1) HeLa, 2) HL60, 3) Jurkat, 4) Ramos and 5) spleen lysate with HLA-B antibody. Predicted molecular weight: ~40/45 kDa (unmodified/glycosylated).
6 / 6
Western blot testing of human 1) HeLa, 2) HL60, 3) A549 and 4) Jurkat lysate with HLA-B antibody. Predicted molecular weight: ~40/45 kDa (unmodified/glycosylated).
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IHC testing of FFPE human tonsil tissue with HLA-B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human tonsil tissue with HLA-B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human spleen tissue with HLA-B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat spleen tissue with HLA-B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HeLa, 2) HL60, 3) Jurkat, 4) Ramos and 5) spleen lysate with HLA-B antibody. Predicted molecular weight: ~40/45 kDa (unmodified/glycosylated).
Western blot testing of human 1) HeLa, 2) HL60, 3) A549 and 4) Jurkat lysate with HLA-B antibody. Predicted molecular weight: ~40/45 kDa (unmodified/glycosylated).

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the HLA-B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Involved in the presentation of foreign antigens to the immune system.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids from the human protein were used as the immunogen for the HLA-B antibody.
Limitation:
This HLA-B antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P30464