CDC25A Antibody (pS124)

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NSJ Bioreagents
Product Code: NSJ-F54292
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54292-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54292-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the phospho-CDC25A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of lysate from human A2780 cells untreated or treated with alkaline phosphatase using phospho-CDC25A antibody. Predicted molecular weight: 59-70 kDa.
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Western blot testing of lysate from human 293 cells untreated or treated with alkaline phosphatase using phospho-CDC25A antibody. Predicted molecular weight: 59-70 kDa.
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IHC testing of FFPE human breast carcinoma tissue with phospho-CDC25A antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
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Western blot testing of lysate from human A2780 cells untreated or treated with alkaline phosphatase using phospho-CDC25A antibody. Predicted molecular weight: 59-70 kDa.
Western blot testing of lysate from human 293 cells untreated or treated with alkaline phosphatase using phospho-CDC25A antibody. Predicted molecular weight: 59-70 kDa.
IHC testing of FFPE human breast carcinoma tissue with phospho-CDC25A antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the phospho-CDC25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
The amino acids surrounding phosphorylated serine 124 from the human protein were used as the immunogen for the phospho-CDC25A antibody.
Limitation:
This Phospho-CDC25A antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P30304