SGPP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54282
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54282-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54282-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the SGPP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human placenta tissue with SGPP1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human kidney tissue with SGPP1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of human 1) HACAT and 2) A431 cell lysate with SGPP1 antibody. Predicted molecular weight ~49 kDa.
4 / 5
Western blot testing of human placenta lysate with SGPP1 antibody. Predicted molecular weight ~49 kDa.
5 / 5
Western blot testing of human NCI-H292 cell lysate with SGPP1 antibody. Predicted molecular weight ~49 kDa.
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IHC testing of FFPE human placenta tissue with SGPP1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with SGPP1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HACAT and 2) A431 cell lysate with SGPP1 antibody. Predicted molecular weight ~49 kDa.
Western blot testing of human placenta lysate with SGPP1 antibody. Predicted molecular weight ~49 kDa.
Western blot testing of human NCI-H292 cell lysate with SGPP1 antibody. Predicted molecular weight ~49 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the SGPP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides [supplied by OMIM].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 13-42 from the human protein were used as the immunogen for the SGPP1 antibody.
Limitation:
This SGPP1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9BX95