Squalene synthase Antibody / SQS / FDFT1

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NSJ Bioreagents
Product Code: NSJ-F54769
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54769-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54769-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Squalene synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC testing of FFPE human hepatocellular carcinoma tissue with Squalene synthase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of human A375 in the absence (left) and presence (right) of immunizing peptide using Squalene synthase antibody. Predicted molecular weight ~48 kDa.
3 / 3
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Squalene synthase antibody.
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IHC testing of FFPE human hepatocellular carcinoma tissue with Squalene synthase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human A375 in the absence (left) and presence (right) of immunizing peptide using Squalene synthase antibody. Predicted molecular weight ~48 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Squalene synthase antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the Squalene synthase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FDFT1 / Squalene synthase catalyzes the first step in the cholesterol biosynthetic pathway, the conversion of trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5-prime to the transcription start site. Sequence analysis of this region shows that it contains a sterol regulatory element-1 (SRE1) previously identified in other sterol regulated genes and 2 putative NF1 binding sites.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 140-170 from the human protein was used as the immunogen for the Squalene synthase antibody.
Limitation:
This Squalene synthase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P37268