CES2 Antibody / Carboxylesterase 2

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NSJ Bioreagents
Product Code: NSJ-F54270
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54270-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54270-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Carboxylesterase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human hepatocarcinoma with Carboxylesterase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Western blot testing of human 1) HepG2 and 2) liver lysate with Carboxylesterase 2 antibody. Predicted molecular weight ~62 kDa.
3 / 5
Western blot testing of human 1) SW620 and 2) liver lysate with Carboxylesterase 2 antibody. Predicted molecular weight ~62 kDa.
4 / 5
Flow cytometry testing of fixed and permeabilized human U-87 MG cells with Carboxylesterase 2 antibody; Blue=isotype control, Green= Carboxylesterase 2 antibody.
5 / 5
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Carboxylesterase 2 antibody; Blue=isotype control, Green= Carboxylesterase 2 antibody.
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IHC testing of FFPE human hepatocarcinoma with Carboxylesterase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HepG2 and 2) liver lysate with Carboxylesterase 2 antibody. Predicted molecular weight ~62 kDa.
Western blot testing of human 1) SW620 and 2) liver lysate with Carboxylesterase 2 antibody. Predicted molecular weight ~62 kDa.
Flow cytometry testing of fixed and permeabilized human U-87 MG cells with Carboxylesterase 2 antibody; Blue=isotype control, Green= Carboxylesterase 2 antibody.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Carboxylesterase 2 antibody; Blue=isotype control, Green= Carboxylesterase 2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Carboxylesterase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CES2 is a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 340-369 from the human protein were used as the immunogen for the Carboxylesterase 2 antibody.
Limitation:
This Carboxylesterase 2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O00748