HEXA Antibody / Hexosaminidase A

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NSJ Bioreagents
Product Code: NSJ-F54305
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54305-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54305-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Hexosaminidase A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human liver tissue with Hexosaminidase A antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human brain lysate with Hexosaminidase A antibody. Expected molecular weight: ~60 kDa (precursor), ~54 kDa (mature).
3 / 4
Western blot testing of human HepG2 cell lysate with Hexosaminidase A antibody. Expected molecular weight: ~60 kDa (precursor), ~54 kDa (mature).
4 / 4
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Hexosaminidase A antibody; Blue=isotype control, Green= Hexosaminidase A antibody.
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IHC testing of FFPE human liver tissue with Hexosaminidase A antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human brain lysate with Hexosaminidase A antibody. Expected molecular weight: ~60 kDa (precursor), ~54 kDa (mature).
Western blot testing of human HepG2 cell lysate with Hexosaminidase A antibody. Expected molecular weight: ~60 kDa (precursor), ~54 kDa (mature).
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Hexosaminidase A antibody; Blue=isotype control, Green= Hexosaminidase A antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Hexosaminidase A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HEXA is the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 142-170 from the human protein were used as the immunogen for the Hexosaminidase A antibody.
Limitation:
This Hexosaminidase A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P06865