HLA-DPB1 Antibody (MHC II)

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NSJ Bioreagents
Product Code: NSJ-F54283
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54283-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54283-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HLA-DPB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human tonsil tissue with HLA-DPB1 antibody.
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IHC testing of FFPE human lymph node tissue with HLA-DPB1 antibody.
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Western blot testing of human Raji cell lysate with HLA-DPB1 antibody. Predicted molecular weight: 29 kDa.
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Western blot testing of human lung lysate with HLA-DPB1 antibody. Predicted molecular weight: 29 kDa.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with HLA-DPB1 antibody. Predicted molecular weight ~29 kDa.
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Flow cytometry testing of fixed and permeabilized human U-2 OS cells with HLA-DPB1 antibody; Blue=isotype control, Green= HLA-DPB1 antibody.
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IHC testing of FFPE human tonsil tissue with HLA-DPB1 antibody.
IHC testing of FFPE human lymph node tissue with HLA-DPB1 antibody.
Western blot testing of human Raji cell lysate with HLA-DPB1 antibody. Predicted molecular weight: 29 kDa.
Western blot testing of human lung lysate with HLA-DPB1 antibody. Predicted molecular weight: 29 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with HLA-DPB1 antibody. Predicted molecular weight ~29 kDa.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with HLA-DPB1 antibody; Blue=isotype control, Green= HLA-DPB1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the HLA-DPB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 77-105 from the human protein were used as the immunogen for the HLA-DPB1 antibody.
Limitation:
This HLA-DPB1 antibody is available for research use only.
Localization:
Cell surface
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P04440