HLA-DRB1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54895
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54895-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54895-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HLA-DRB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) NCI-H1299 and 2) Raji cell lysate with HLA-DRB1 antibody. Predicted molecular weight ~30 kDa.
2 / 3
IHC testing of FFPE human tonsil tissue with HLA-DRB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
Flow cytometry testing of human WiDr cells with HLA-DRB1 antibody; Blue=isotype control, Green= HLA-DRB1 antibody.
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Western blot testing of human 1) NCI-H1299 and 2) Raji cell lysate with HLA-DRB1 antibody. Predicted molecular weight ~30 kDa.
IHC testing of FFPE human tonsil tissue with HLA-DRB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human WiDr cells with HLA-DRB1 antibody; Blue=isotype control, Green= HLA-DRB1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the HLA-DRB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 30-58 from the human protein was used as the immunogen for the HLA-DRB1 antibody.
Limitation:
This HLA-DRB1 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P04229