PLA2G7 Antibody / Lp-PLA2 / PAFAH

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NSJ Bioreagents
Product Code: NSJ-F54313
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54313-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54313-0.4ML0.4 ml£469.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Lp-PLA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human tonsil tissue with Lp-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human tonsil tissue with Lp-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 6
IHC testing of FFPE human colon tissue with Lp-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 6
Western blot testing of human 1) liver, 2) lung, 3) spleen and 4) plasma lysate with Lp-PLA2 antibody. Expected molecular weight: 45-67 kDa depending on glycosylation level.
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Western blot testing of human HL60 cell lysate with Lp-PLA2 antibody. Expected molecular weight: 45-67 kDa depending on glycosylation level.
6 / 6
Flow cytometry testing of fixed and permeabilized human HL60 cells with Lp-PLA2 antibody; Blue=isotype control, Green= Lp-PLA2 antibody.
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IHC testing of FFPE human tonsil tissue with Lp-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human tonsil tissue with Lp-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon tissue with Lp-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) liver, 2) lung, 3) spleen and 4) plasma lysate with Lp-PLA2 antibody. Expected molecular weight: 45-67 kDa depending on glycosylation level.
Western blot testing of human HL60 cell lysate with Lp-PLA2 antibody. Expected molecular weight: 45-67 kDa depending on glycosylation level.
Flow cytometry testing of fixed and permeabilized human HL60 cells with Lp-PLA2 antibody; Blue=isotype control, Green= Lp-PLA2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Lp-PLA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 200-228 from the human protein was used as the immunogen for the Lp-PLA2 antibody.
Limitation:
This Lp-PLA2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q13093