Ras-interacting protein 1 Antibody / RASIP1

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NSJ Bioreagents
Product Code: NSJ-F54892
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54892-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54892-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Ras-interacting protein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) HepG2, 2) HUVEC, 3) Jurkat and 4) K562 cell lysate with Ras-interacting protein 1 antibody. Predicted molecular weight ~103 kDa.
2 / 3
IHC testing of FFPE human brain tissue with Ras-interacting protein 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
Flow cytometry testing of fixed and permeabilized human K562 cells with Ras-interacting protein 1 antibody; Blue=isotype control, Green= Ras-interacting protein 1 antibody.
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Western blot testing of human 1) HepG2, 2) HUVEC, 3) Jurkat and 4) K562 cell lysate with Ras-interacting protein 1 antibody. Predicted molecular weight ~103 kDa.
IHC testing of FFPE human brain tissue with Ras-interacting protein 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human K562 cells with Ras-interacting protein 1 antibody; Blue=isotype control, Green= Ras-interacting protein 1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Ras-interacting protein 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
RASIP1 may act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 651-678 from the human protein was used as the immunogen for the Ras-interacting protein 1 antibody.
Limitation:
This Ras-interacting protein 1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q5U651