SLC22A2 Antibody / OCT2

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NSJ Bioreagents
Product Code: NSJ-F54507
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54507-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54507-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the SLC22A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human stomach tissue with SLC22A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human kidney tissue with SLC22A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) HEK293, 2) A431, 3) HepG2, 4) Jurkat and 5) Raji cell lysate with SLC22A2 antibody. Expected molecular weight: 55-100 kDa depending on glycosylation level.
4 / 4
Flow cytometry testing of fixed and permeabilized human A431 cells with SLC22A2 antibody; Blue=isotype control, Green= SLC22A2 antibody.
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IHC testing of FFPE human stomach tissue with SLC22A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with SLC22A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HEK293, 2) A431, 3) HepG2, 4) Jurkat and 5) Raji cell lysate with SLC22A2 antibody. Expected molecular weight: 55-100 kDa depending on glycosylation level.
Flow cytometry testing of fixed and permeabilized human A431 cells with SLC22A2 antibody; Blue=isotype control, Green= SLC22A2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the SLC22A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mediates tubular uptake of organic compounds from circulation. Mediates the influx of agmatine, dopamine, noradrenaline (norepinephrine), serotonin, choline, famotidine, ranitidine, histamin, creatinine, amantadine, memantine, acriflavine, 4-[4-(dimethylamino)-styryl]-N-methylpyridinium ASP, amiloride, metformin, N-1-methylnicotinamide (NMN), tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, cisplatin and oxaliplatin. Cisplatin may develop a nephrotoxic action. Transport of creatinine is inhibited by fluoroquinolones such as DX-619 and LVFX. This transporter is a major determinant of the anticancer activity of oxaliplatin and may contribute to antitumor specificity.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the SLC22A2 antibody.
Limitation:
This SLC22A2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O15244