SPNS2 Antibody / Protein spinster homolog 2

NSJ Bioreagents
Product Code: NSJ-F54287
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54287-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54287-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the SPNS2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Western blot testing of human 1) heart, 2) kidney, 3) skeletal muscle and 4) SK-BR-3 lysate with SPNS2 antibody. Predicted molecular weight ~58 kDa.
2 / 6
Western blot testing of human 1) brain, 2) 293, and 3) SK-BR-3 lysate with SPNS2 antibody. Predicted molecular weight ~58 kDa.
3 / 6
Western blot testing of human MDA-MB-231 lysate with SPNS2 antibody. Predicted molecular weight ~58 kDa.
4 / 6
Western blot testing of human SK-OV-3 lysate with SPNS2 antibody. Predicted molecular weight ~58 kDa.
5 / 6
IHC testing of FFPE human liver tissue with SPNS2 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
6 / 6
Flow cytometry testing of fixed and permeabilized human SK-OV-3 cells with SPNS2 antibody; Blue=isotype control, Green= SPNS2 antibody.

Western blot testing of human 1) heart, 2) kidney, 3) skeletal muscle and 4) SK-BR-3 lysate with SPNS2 antibody. Predicted molecular weight ~58 kDa.
Western blot testing of human 1) brain, 2) 293, and 3) SK-BR-3 lysate with SPNS2 antibody. Predicted molecular weight ~58 kDa.
Western blot testing of human MDA-MB-231 lysate with SPNS2 antibody. Predicted molecular weight ~58 kDa.
Western blot testing of human SK-OV-3 lysate with SPNS2 antibody. Predicted molecular weight ~58 kDa.
IHC testing of FFPE human liver tissue with SPNS2 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human SK-OV-3 cells with SPNS2 antibody; Blue=isotype control, Green= SPNS2 antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the SPNS2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 68-94 from the human protein were used as the immunogen for the SPNS2 antibody.
Limitation:
This SPNS2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q8IVW8