U2AF1 Antibody / U2AF35

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NSJ Bioreagents
Product Code: NSJ-F54776
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54776-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54776-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the U2AF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lung carcinoma tissue with U2AF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of Ramos cell lysate with U2AF1 antibody. Expected molecular weight: 28-35 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with U2AF1 antibody. Expected molecular weight: 28-35 kDa.
4 / 4
Flow cytometry testing of human Ramos cells with U2AF1 antibody; Blue=isotype control, Green= U2AF1 antibody.
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IHC testing of FFPE human lung carcinoma tissue with U2AF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of Ramos cell lysate with U2AF1 antibody. Expected molecular weight: 28-35 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with U2AF1 antibody. Expected molecular weight: 28-35 kDa.
Flow cytometry testing of human Ramos cells with U2AF1 antibody; Blue=isotype control, Green= U2AF1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the U2AF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 99-125 from the human protein was used as the immunogen for the U2AF1 antibody.
Limitation:
This U2AF1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q01081