VLDLR Antibody / VLDL Receptor

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NSJ Bioreagents
Product Code: NSJ-F54296
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54296-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54296-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the VLDL Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human heart tissue with VLDL Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human heart tissue with VLDL Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 6
IHC testing of FFPE human skeletal muscle tissue with VLDL Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 6
Western blot testing of human 293T cell lysate with VLDL Receptor antibody. Expected molecular weight: 96-160 kDa depending on glycosylation level.
5 / 6
Western blot testing of human heart lysate with VLDL Receptor antibody. Expected molecular weight: 96-160 kDa depending on glycosylation level.
6 / 6
Flow cytometry testing of fixed and permeabilized human ThP-1 cells with VLDL Receptor antibody; Blue=isotype control, Green= VLDL Receptor antibody.
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IHC testing of FFPE human heart tissue with VLDL Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human heart tissue with VLDL Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human skeletal muscle tissue with VLDL Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 293T cell lysate with VLDL Receptor antibody. Expected molecular weight: 96-160 kDa depending on glycosylation level.
Western blot testing of human heart lysate with VLDL Receptor antibody. Expected molecular weight: 96-160 kDa depending on glycosylation level.
Flow cytometry testing of fixed and permeabilized human ThP-1 cells with VLDL Receptor antibody; Blue=isotype control, Green= VLDL Receptor antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the VLDL Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 484-510 from the human protein were used as the immunogen for the VLDL Receptor antibody.
Limitation:
This VLDL Receptor antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P98155