DLL3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54277
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54277-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54277-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the DLL3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human thyroid carcinoma tissue with DLL3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human brain tissue with DLL3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of fixed and permeabilized human 293 cells with DLL3 antibody (green) and DAPI nuclear stain (blue).
4 / 5
Western blot testing of human 1) 293, 2) A549, 3) brain, 4) SH-SY5Y and 5) U-2 OS lysate with DLL3 antibody. Predicted molecular weight: ~65 kDa.
5 / 5
Western blot testing of human 1) TT and 2) brain lysate with DLL3 antibody. Predicted molecular weight: ~65 kDa.
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IHC testing of FFPE human thyroid carcinoma tissue with DLL3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with DLL3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human 293 cells with DLL3 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human 1) 293, 2) A549, 3) brain, 4) SH-SY5Y and 5) U-2 OS lysate with DLL3 antibody. Predicted molecular weight: ~65 kDa.
Western blot testing of human 1) TT and 2) brain lysate with DLL3 antibody. Predicted molecular weight: ~65 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the DLL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 545-573 from the human protein were used as the immunogen for the DLL3 antibody.
Limitation:
This DLL3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9NYJ7