KIF22 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54399
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54399-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54399-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the KIF22 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) MCF7, 2) HeLa, 3) SH-SY5Y, 4) Jurkat and 5) HL60 cell lysate with KIF22 antibody. Predicted molecular weight ~73 kDa.
2 / 4
IHC testing of FFPE human kidney tissue with KIF22 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
IHC testing of FFPE human skin tissue with KIF22 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with KIF22 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
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Western blot testing of human 1) MCF7, 2) HeLa, 3) SH-SY5Y, 4) Jurkat and 5) HL60 cell lysate with KIF22 antibody. Predicted molecular weight ~73 kDa.
IHC testing of FFPE human kidney tissue with KIF22 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human skin tissue with KIF22 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with KIF22 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the KIF22 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 423-456 from the human protein was used as the immunogen for the KIF22 antibody.
Limitation:
This KIF22 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q14807