ATP1A2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54297
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54297-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54297-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ATP1A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human skeletal muscle lysate with ATP1A2 antibody. Predicted molecular weight ~112 kDa.
2 / 5
Western blot testing of human MCF7 cell lysate with ATP1A2 antibody. Predicted molecular weight ~112 kDa.
3 / 5
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with ATP1A2 antibody (green) and DAPI nuclear stain (blue).
4 / 5
IHC testing of FFPE human brain tissue with ATP1A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of fixed and permeabilized human MCF7 cells with ATP1A2 antibody; Blue=isotype control, Green= ATP1A2 antibody.
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Western blot testing of human skeletal muscle lysate with ATP1A2 antibody. Predicted molecular weight ~112 kDa.
Western blot testing of human MCF7 cell lysate with ATP1A2 antibody. Predicted molecular weight ~112 kDa.
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with ATP1A2 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human brain tissue with ATP1A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with ATP1A2 antibody; Blue=isotype control, Green= ATP1A2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the ATP1A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 451-479 from the human protein were used as the immunogen for the ATP1A2 antibody.
Limitation:
This ATP1A2 antibody is available for research use only.
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
P50993