BCKDHB Antibody

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NSJ Bioreagents
Product Code: NSJ-F54271
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54271-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54271-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the BCKDHB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Western blot testing of human 1) HeLa, 2) 293T and 3) Jurkat cell lysate with BCKDHB antibody. Predicted molecular weight: ~43 kDa, observed molecular weight: ~35 kDa.
2 / 6
Western blot testing of human 1) HeLa, 2) Jurkat and 3) Daudi cell lysate with BCKDHB antibody. Predicted molecular weight: ~43 kDa, observed molecular weight: ~35 kDa.
3 / 6
IHC testing of FFPE human kidney tissue with BCKDHB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 6
IHC testing of FFPE human hepatocarinoma with BCKDHB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 6
Immunofluorescent staining of fixed and permeabilized human HeLa cells with BCKDHB antibody (green) and DAPI nuclear stain (blue).
6 / 6
Flow cytometry testing of fixed and permeabilized human HeLa cells with BCKDHB antibody; Blue=isotype control, Green= BCKDHB antibody.
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Western blot testing of human 1) HeLa, 2) 293T and 3) Jurkat cell lysate with BCKDHB antibody. Predicted molecular weight: ~43 kDa, observed molecular weight: ~35 kDa.
Western blot testing of human 1) HeLa, 2) Jurkat and 3) Daudi cell lysate with BCKDHB antibody. Predicted molecular weight: ~43 kDa, observed molecular weight: ~35 kDa.
IHC testing of FFPE human kidney tissue with BCKDHB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human hepatocarinoma with BCKDHB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with BCKDHB antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of fixed and permeabilized human HeLa cells with BCKDHB antibody; Blue=isotype control, Green= BCKDHB antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the BCKDHB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Branched-chain keto acid dehydrogenase is a multienzyme complex associated with the inner membrane of mitochondria, and functions in the catabolism of branched-chain amino acids. The complex consists of multiple copies of 3 components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). This gene encodes the E1 beta subunit, and mutations therein have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation, and feeding problems.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 41-70 from the human protein were used as the immunogen for the BCKDHB antibody.
Limitation:
This BCKDHB antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P21953