Beta Catenin Antibody

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NSJ Bioreagents
Product Code: NSJ-F54317
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54317-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54317-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Catenin Beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of fixed and permeabilized human T-47D cells with Catenin Beta antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
2 / 6
Immunofluorescent staining of fixed and permeabilized human 293 cells with Catenin Beta antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 6
IHC testing of FFPE human stomach tissue with Catenin Beta antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 6
IHC testing of FFPE human colon carcinoma tissue with Catenin Beta antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 6
Western blot testing of human 1) 293 and 2) T-47D cell lysate with Catenin Beta antibody. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
6 / 6
Flow cytometry testing of fixed and permeabilized human 293 cells with Catenin Beta antibody; Blue=isotype control, Green= Catenin Beta antibody.
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Immunofluorescent staining of fixed and permeabilized human T-47D cells with Catenin Beta antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Immunofluorescent staining of fixed and permeabilized human 293 cells with Catenin Beta antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human stomach tissue with Catenin Beta antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon carcinoma tissue with Catenin Beta antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) 293 and 2) T-47D cell lysate with Catenin Beta antibody. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
Flow cytometry testing of fixed and permeabilized human 293 cells with Catenin Beta antibody; Blue=isotype control, Green= Catenin Beta antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Catenin Beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 692-721 from the human protein was used as the immunogen for the Catenin Beta antibody.
Limitation:
This Catenin Beta antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P35222