Fetuin-A Antibody / AHSG

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NSJ Bioreagents
Product Code: NSJ-F54268
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54268-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54268-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Fetuin-A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) blood plasma and 2) liver lysate with AHSG antibody. Predicted molecular weight: ~39/45-59 kDa (unmodified/glycosylated).
2 / 5
IHC testing of FFPE human liver tissue with Fetuin-A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Fetuin-A antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 5
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Fetuin-A antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
5 / 5
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Fetuin-A antibody; Blue=isotype control, Green= Fetuin-A antibody.
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Western blot testing of human 1) blood plasma and 2) liver lysate with AHSG antibody. Predicted molecular weight: ~39/45-59 kDa (unmodified/glycosylated).
IHC testing of FFPE human liver tissue with Fetuin-A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Fetuin-A antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Fetuin-A antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Fetuin-A antibody; Blue=isotype control, Green= Fetuin-A antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Fetuin-A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 247-276 from the human protein were used as the immunogen for the Fetuin-A antibody.
Limitation:
This Fetuin-A antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P02765