Glucagon Antibody

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NSJ Bioreagents
Product Code: NSJ-F54303
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54303-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54303-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Glucagon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human pancreas tissue with Glucagon antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human liver tissue with Glucagon antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of FFPE human pancreas with Glucagon antibody (green) and DAPI nuclear stain (blue).
4 / 5
Western blot testing of human 1) PANC1, 2) brain and 3) Jurkat lysate with Glucagon antibody. Predicted molecular weight ~21 kDa (pro form).
5 / 5
Flow cytometry testing of fixed and permeabilized human Jurkat cells with Glucagon antibody; Blue=isotype control, Green= Glucagon antibody.
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IHC testing of FFPE human pancreas tissue with Glucagon antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with Glucagon antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Immunofluorescent staining of FFPE human pancreas with Glucagon antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human 1) PANC1, 2) brain and 3) Jurkat lysate with Glucagon antibody. Predicted molecular weight ~21 kDa (pro form).
Flow cytometry testing of fixed and permeabilized human Jurkat cells with Glucagon antibody; Blue=isotype control, Green= Glucagon antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Glucagon antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glucagon is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 119-148 from the human protein were used as the immunogen for the Glucagon antibody.
Limitation:
This Glucagon antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P01275