GLO1 Antibody / Glyoxalase I

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NSJ Bioreagents
Product Code: NSJ-F54301
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54301-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54301-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Glyoxalase I antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with Glyoxalase I antibody. Predicted molecular weight ~21 kDa.
2 / 5
Western blot testing of human HL60 cell lysate with Glyoxalase I antibody. Predicted molecular weight ~21 kDa.
3 / 5
IHC testing of FFPE human brain tissue with Glyoxalase I antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Immunofluorescent staining of fixed and permeabilized human WiDr cells with Glyoxalase I antibody (green) and DAPI nuclear stain (blue).
5 / 5
Flow cytometry testing of fixed and permeabilized human WiDr cells with Glyoxalase I antibody; Blue=isotype control, Green= Glyoxalase I antibody.
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Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with Glyoxalase I antibody. Predicted molecular weight ~21 kDa.
Western blot testing of human HL60 cell lysate with Glyoxalase I antibody. Predicted molecular weight ~21 kDa.
IHC testing of FFPE human brain tissue with Glyoxalase I antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human WiDr cells with Glyoxalase I antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of fixed and permeabilized human WiDr cells with Glyoxalase I antibody; Blue=isotype control, Green= Glyoxalase I antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Glyoxalase I antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The enzyme GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 23-51 from the human protein were used as the immunogen for the Glyoxalase I antibody.
Limitation:
This Glyoxalase I antibody is available for research use only.
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
Q04760