Inhibin beta A chain Antibody / INHBA

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NSJ Bioreagents
Product Code: NSJ-F54579
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54579-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54579-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Inhibin beta A chain antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human stomach tissue with Inhibin beta A chain antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HepG2 cells with Inhibin beta A chain antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human CEM cell lysate with Inhibin beta A chain antibody. Predicted molecular weight ~47 kDa.
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Flow cytometry testing of human CEM cells with Inhibin beta A chain antibody; Blue=isotype control, Green= Inhibin beta A chain antibody.
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IHC testing of FFPE human stomach tissue with Inhibin beta A chain antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HepG2 cells with Inhibin beta A chain antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human CEM cell lysate with Inhibin beta A chain antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human CEM cells with Inhibin beta A chain antibody; Blue=isotype control, Green= Inhibin beta A chain antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Inhibin beta A chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 85-112 from the human protein was used as the immunogen for the Inhibin beta A chain antibody.
Limitation:
This Inhibin beta A chain antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P08476