M-CSF Antibody / Macrophage Colony Stimulating Factor 1 / CSF1

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NSJ Bioreagents
Product Code: NSJ-F54882
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54882-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54882-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the M-CSF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human kidney tissue with M-CSF antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Immunofluorescent staining of human MDA-MB-231 cells with M-CSF antibody (green) and DAPI nuclear stain (blue).
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4 / 4
Flow cytometry testing of human HEK293 cells with M-CSF antibody; Blue=isotype control, Green= M-CSF antibody.
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IHC testing of FFPE human kidney tissue with M-CSF antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MDA-MB-231 cells with M-CSF antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human HEK293 cells with M-CSF antibody; Blue=isotype control, Green= M-CSF antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the M-CSF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
M-CSF is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. This protein may be involved in development of the placenta.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 230-257 from the human protein was used as the immunogen for the M-CSF antibody.
Limitation:
This M-CSF antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P09603