RACGAP1 Antibody

NSJ Bioreagents
Product Code: NSJ-F54599
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54599-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54599-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Rac GTPase-activating protein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human testis tissue with Rac GTPase-activating protein 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human MCF7 cells with Rac GTPase-activating protein 1 antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human MCF7 cell lysate with Rac GTPase-activating protein 1 antibody. Predicted molecular weight ~71 kDa.
4 / 4
Flow cytometry testing of human MCF7 cells with Rac GTPase-activating protein 1 antibody; Blue=isotype control, Green= Rac GTPase-activating protein 1 antibody.

IHC testing of FFPE human testis tissue with Rac GTPase-activating protein 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MCF7 cells with Rac GTPase-activating protein 1 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human MCF7 cell lysate with Rac GTPase-activating protein 1 antibody. Predicted molecular weight ~71 kDa.
Flow cytometry testing of human MCF7 cells with Rac GTPase-activating protein 1 antibody; Blue=isotype control, Green= Rac GTPase-activating protein 1 antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Rac GTPase-activating protein 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC, and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 23-50 from the human protein was used as the immunogen for the Rac GTPase-activating protein 1 antibody.
Limitation:
This Rac GTPase-activating protein 1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9H0H5