Arginyl-tRNA synthetase Antibody (cytoplasmic) / RARS1

NSJ Bioreagents
Product Code: NSJ-F54575
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54575-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54575-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the RARS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human MCF7 cell lysate with RARS antibody. Predicted molecular weight ~75 kDa.
2 / 4
IHC testing of FFPE human brain tissue with RARS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human MCF7 cells with RARS antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human MCF7 cells with RARS antibody; Blue=isotype control, Green= RARS antibody.

Western blot testing of human MCF7 cell lysate with RARS antibody. Predicted molecular weight ~75 kDa.
IHC testing of FFPE human brain tissue with RARS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MCF7 cells with RARS antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human MCF7 cells with RARS antibody; Blue=isotype control, Green= RARS antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the RARS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 605-634 from the human protein was used as the immunogen for the RARS antibody.
Limitation:
This RARS antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P54136