Thymidylate Synthase Antibody

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NSJ Bioreagents
Product Code: NSJ-F54315
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54315-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54315-0.4ML0.4 ml£469.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Thymidylate Synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Western blot testing of human 1) HeLa, 2) Jurkat and 3) HL60 cell lysate with Thymidylate Synthase antibody. Predicted molecular weight ~36 kDa.
2 / 6
IHC testing of FFPE human colon carcinoma tissue with IRF7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 6
IHC testing of FFPE human breast carcinoma tissue with IRF7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 6
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Thymidylate Synthase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
5 / 6
Immunofluorescent staining of fixed and permeabilized human WiDr cells with Thymidylate Synthase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
6 / 6
Flow cytometry testing of fixed and permeabilized human HeLa cells with Thymidylate Synthase antibody; Blue=isotype control, Green= Thymidylate Synthase antibody.
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Western blot testing of human 1) HeLa, 2) Jurkat and 3) HL60 cell lysate with Thymidylate Synthase antibody. Predicted molecular weight ~36 kDa.
IHC testing of FFPE human colon carcinoma tissue with IRF7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human breast carcinoma tissue with IRF7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Thymidylate Synthase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Immunofluorescent staining of fixed and permeabilized human WiDr cells with Thymidylate Synthase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human HeLa cells with Thymidylate Synthase antibody; Blue=isotype control, Green= Thymidylate Synthase antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Thymidylate Synthase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 265-294 from the human protein was used as the immunogen for the Thymidylate Synthase Antibody.
Limitation:
This Thymidylate Synthase antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P04818