KLF11 Antibody / TIEG2

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NSJ Bioreagents
Product Code: NSJ-F54867
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54867-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54867-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the KLF11 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human K562 cell lysate with KLF11 antibody. Expected molecular weight: 55-65 kDa.
2 / 4
Immunofluorescent staining of human HeLa cells with KLF11 antibody (green) and DAPI nuclear stain (blue).
3 / 4
IHC testing of FFPE human brain tissue with KLF11 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HEK293 cells with KLF11 antibody; Blue=isotype control, Green= KLF11 antibody.
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Western blot testing of human K562 cell lysate with KLF11 antibody. Expected molecular weight: 55-65 kDa.
Immunofluorescent staining of human HeLa cells with KLF11 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human brain tissue with KLF11 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with KLF11 antibody; Blue=isotype control, Green= KLF11 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the KLF11 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TIEG2 is a transcription factor. The protein activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. It represses transcription of SMAD7 which enhances TGF-beta signaling. It induces apoptosis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 7-36 from the human protein was used as the immunogen for the KLF11 antibody.
Limitation:
This KLF11 antibody is available for research use only.
Localization:
Nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
O14901