Beta-secretase 1 Antibody / Memapsin-2 / BACE1C

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NSJ Bioreagents
Product Code: NSJ-F54840
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54840-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54840-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Beta-secretase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human NCI-H460 cell lysate with Beta-secretase 1 antibody. Predicted molecular weight ~56 kDa.
2 / 3
IHC testing of FFPE human cancer tissue with Beta-secretase 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
Flow cytometry testing of human NCI-H460 cells with Beta-secretase 1 antibody; Blue=isotype control, Green= Beta-secretase 1 antibody.
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Western blot testing of human NCI-H460 cell lysate with Beta-secretase 1 antibody. Predicted molecular weight ~56 kDa.
IHC testing of FFPE human cancer tissue with Beta-secretase 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human NCI-H460 cells with Beta-secretase 1 antibody; Blue=isotype control, Green= Beta-secretase 1 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Beta-secretase 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the BACE1. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 125-154 from the human protein was used as the immunogen for the Beta-secretase 1 antibody.
Limitation:
This Beta-secretase 1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P56817