IMMT Antibody / MIC60

NSJ Bioreagents
Product Code: NSJ-F54800
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54800-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54800-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the IMMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human colon carcinoma tissue with IMMT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with IMMT antibody.
3 / 4
Western blot testing of human HeLa cell lysate with IMMT antibody. Predicted molecular weight ~84 kDa.
4 / 4
Flow cytometry testing of human HeLa cells with IMMT antibody; Blue=isotype control, Green= IMMT antibody.

IHC testing of FFPE human colon carcinoma tissue with IMMT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with IMMT antibody.
Western blot testing of human HeLa cell lysate with IMMT antibody. Predicted molecular weight ~84 kDa.
Flow cytometry testing of human HeLa cells with IMMT antibody; Blue=isotype control, Green= IMMT antibody.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the IMMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mitochondria are the center of cellular energy production and essential metabolic reactions. As double membrane-bound organelles, mitochondria from different species, tissues, and metabolic states are highly polymorphic in nature, yet exhibit common structural features. The ultrastructural variations in mitochondrial architecture occur mainly due to the differences in the amount and shape of cristae. Abundant cristae are found in mitochondria from tissues where energy demand is high. Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins. It appears to play an important role in the maintenance of cristae morphology.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 700-729 from the human protein was used as the immunogen for the IMMT antibody.
Limitation:
This IMMT antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q16891