Major Vault Protein Antibody / MVP

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NSJ Bioreagents
Product Code: NSJ-F54845
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54845-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54845-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Major Vault Protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lung carcinoma tissue with Major Vault Protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Major Vault Protein antibody.
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Western blot testing of human A375 cell lysate with Major Vault Protein antibody. Expected molecular weight: 104~110 kDa.
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Flow cytometry testing of human NCI-H292 cells with Major Vault Protein antibody; Blue=isotype control, Green= Major Vault Protein antibody.
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IHC testing of FFPE human lung carcinoma tissue with Major Vault Protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Major Vault Protein antibody.
Western blot testing of human A375 cell lysate with Major Vault Protein antibody. Expected molecular weight: 104~110 kDa.
Flow cytometry testing of human NCI-H292 cells with Major Vault Protein antibody; Blue=isotype control, Green= Major Vault Protein antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Major Vault Protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MVP, the major vault protein, is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 863-893 from the human protein was used as the immunogen for the Major Vault Protein antibody.
Limitation:
This Major Vault Protein antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q14764