Peroxiredoxin 1 Antibody / PRDX1

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NSJ Bioreagents
Product Code: NSJ-F54796
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54796-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54796-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Peroxiredoxin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Peroxiredoxin 1 antibody.
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Western blot testing of human CCRF-CEM cell lysate with Peroxiredoxin 1 antibody. Predicted molecular weight ~22 kDa.
3 / 4
IHC testing of FFPE human colon carcinoma tissue with Peroxiredoxin 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HEK293 cells with Peroxiredoxin 1 antibody; Blue=isotype control, Green= Peroxiredoxin 1 antibody.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Peroxiredoxin 1 antibody.
Western blot testing of human CCRF-CEM cell lysate with Peroxiredoxin 1 antibody. Predicted molecular weight ~22 kDa.
IHC testing of FFPE human colon carcinoma tissue with Peroxiredoxin 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with Peroxiredoxin 1 antibody; Blue=isotype control, Green= Peroxiredoxin 1 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Peroxiredoxin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PRDX1 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 88-116 from the human protein was used as the immunogen for the Peroxiredoxin 1 antibody.
Limitation:
This Peroxiredoxin 1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q06830