AGAP4 Antibody / AGAP8

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NSJ Bioreagents
Product Code: NSJ-F54390
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54390-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54390-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the AGAP4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human HT-1080 cell lysate with AGAP4 antibody. Predicted molecular weight ~73 kDa.
2 / 5
IHC testing of FFPE human liver tissue with AGAP4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
IHC testing of FFPE mouse liver tissue with AGAP4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Immunofluorescent staining of fixed and permeabilized human HeLa cells with AGAP4 antibody (green) and anti-Actin (red).
5 / 5
Flow cytometry testing of fixed and permeabilized human MCF7 cells with AGAP4 antibody; Blue=isotype control, Green= AGAP4 antibody.
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Western blot testing of human HT-1080 cell lysate with AGAP4 antibody. Predicted molecular weight ~73 kDa.
IHC testing of FFPE human liver tissue with AGAP4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse liver tissue with AGAP4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with AGAP4 antibody (green) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human MCF7 cells with AGAP4 antibody; Blue=isotype control, Green= AGAP4 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the AGAP4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Putative GTPase-activating protein (Potential).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 498-531 from the human protein was used as the immunogen for the AGAP4 antibody.
Limitation:
This AGAP4 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q96P64