R-Cadherin Antibody / CDH4

Contact us
NSJ Bioreagents
Product Code: NSJ-F54374
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54374-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54374-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Cadherin 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) A431 and 2) SH-SY5Y cell lysate with Cadherin 4 antibody. Expected molecular weight: 100-140 kDa depending on glycosylation level.
2 / 5
Western blot testing of human HepG2 cell lysate with Cadherin 4 antibody. Expected molecular weight: 100-140 kDa depending on glycosylation level.
3 / 5
IHC testing of FFPE human brain tissue with Cadherin 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Immunofluorescent staining of human brain tissue with Cadherin 4 antibody (green) and Propidium iodide (red).
5 / 5
Flow cytometry testing of human HepG2 cells with Cadherin 4 antibody; Blue=isotype control, Green= Cadherin 4 antibody.
❮❯

Western blot testing of human 1) A431 and 2) SH-SY5Y cell lysate with Cadherin 4 antibody. Expected molecular weight: 100-140 kDa depending on glycosylation level.
Western blot testing of human HepG2 cell lysate with Cadherin 4 antibody. Expected molecular weight: 100-140 kDa depending on glycosylation level.
IHC testing of FFPE human brain tissue with Cadherin 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human brain tissue with Cadherin 4 antibody (green) and Propidium iodide (red).
Flow cytometry testing of human HepG2 cells with Cadherin 4 antibody; Blue=isotype control, Green= Cadherin 4 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Cadherin 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CDH4 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 175-203 from the human protein was used as the immunogen for the Cadherin 4 antibody.
Limitation:
This Cadherin 4 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P55283