ATG4D Antibody

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NSJ Bioreagents
Product Code: NSJ-F54718
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54718-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54718-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Autophagin 4D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human Y79 cell lysate with Autophagin 4D antibody. Expected molecular weight: 53-56 kDa.
2 / 4
Western blot testing of 1) monkey COS7, 2) human HEK293, 3) MEF (mouse embryonic fibroblasts) and 4) human HeLa cell lysate with Autophagin 4D antibody. Expected molecular weight: 53-56 kDa. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
3 / 4
Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with Autophagin 4D antibody (green) and Hoechst 33342 nuclear stain (blue).
4 / 4
IHC testing of FFPE human cancer tissue with Autophagin 4D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human Y79 cell lysate with Autophagin 4D antibody. Expected molecular weight: 53-56 kDa.
Western blot testing of 1) monkey COS7, 2) human HEK293, 3) MEF (mouse embryonic fibroblasts) and 4) human HeLa cell lysate with Autophagin 4D antibody. Expected molecular weight: 53-56 kDa. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with Autophagin 4D antibody (green) and Hoechst 33342 nuclear stain (blue).
IHC testing of FFPE human cancer tissue with Autophagin 4D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Autophagin 4D antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 441-470 from the human protein was used as the immunogen for the Autophagin 4D antibody.
Limitation:
This Autophagin 4D antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q86TL0