CARD6 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54469
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54469-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54469-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CARD6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human WiDr cell lysate with CARD6 antibody. Predicted molecular weight ~116 kDa.
2 / 4
Western blot testing of human MDA-MB-231 cell lysate with CARD6 antibody. Predicted molecular weight ~116 kDa.
3 / 4
IHC testing of FFPE human skeletal muscle tissue with CARD6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
IHC testing of FFPE human colon tissue with CARD6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human WiDr cell lysate with CARD6 antibody. Predicted molecular weight ~116 kDa.
Western blot testing of human MDA-MB-231 cell lysate with CARD6 antibody. Predicted molecular weight ~116 kDa.
IHC testing of FFPE human skeletal muscle tissue with CARD6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon tissue with CARD6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the CARD6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CARD6 is a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 755-787 from the human protein was used as the immunogen for the CARD6 antibody.
Limitation:
This CARD6 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9BX69