EIF4B Antibody

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NSJ Bioreagents
Product Code: NSJ-F54732
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54732-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54732-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the EIF4B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human HeLa cell lysate with EIF3B antibody. Predicted molecular weight ~69 kDa.
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Western blot testing of human K562 cell lysate with EIF3B antibody. Predicted molecular weight ~69 kDa.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with EIF4B antibody.
4 / 4
IHC testing of FFPE human breast cancer tissue with EIF4B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HeLa cell lysate with EIF3B antibody. Predicted molecular weight ~69 kDa.
Western blot testing of human K562 cell lysate with EIF3B antibody. Predicted molecular weight ~69 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with EIF4B antibody.
IHC testing of FFPE human breast cancer tissue with EIF4B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the EIF4B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 546-575 from the human protein was used as the immunogen for the EIF4B antibody.
Limitation:
This EIF4B antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P23588