GPR45 Antibody

NSJ Bioreagents
Product Code: NSJ-F54644
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54644-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54644-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the GPR45 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human brain tissue with GPR45 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human 1) SH-SY5Y and 2) U-87 MG cell lysate with GPR45 antibody. Predicted molecular weight ~42 kDa.
3 / 4
Western blot testing of human cerebellum tissue lysate with GPR45 antibody. Predicted molecular weight ~42 kDa.
4 / 4
Western blot testing of human MDA-MB-435 cell lysate with GPR45 antibody. Predicted molecular weight ~42 kDa.

IHC testing of FFPE human brain tissue with GPR45 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) SH-SY5Y and 2) U-87 MG cell lysate with GPR45 antibody. Predicted molecular weight ~42 kDa.
Western blot testing of human cerebellum tissue lysate with GPR45 antibody. Predicted molecular weight ~42 kDa.
Western blot testing of human MDA-MB-435 cell lysate with GPR45 antibody. Predicted molecular weight ~42 kDa.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the GPR45 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This intronless gene encodes a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. This protein may function in the central nervous system.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 121-149 from the human protein was used as the immunogen for the GPR45 antibody.
Limitation:
This GPR45 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9Y5Y3