OTUD3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54570
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54570-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54570-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the OTUD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human brain tissue with OTUD3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human testis tissue with OTUD3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human Caco-2 cell lysate with OTUD3 antibody. Predicted molecular weight ~45 kDa.
4 / 4
Western blot testing of human 1) LNCaP, 2) RD, 3) HEK293 and 4) U-251 MG cell lysate with OTUD3 antibody. Predicted molecular weight ~45 kDa.
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IHC testing of FFPE human brain tissue with OTUD3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human testis tissue with OTUD3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human Caco-2 cell lysate with OTUD3 antibody. Predicted molecular weight ~45 kDa.
Western blot testing of human 1) LNCaP, 2) RD, 3) HEK293 and 4) U-251 MG cell lysate with OTUD3 antibody. Predicted molecular weight ~45 kDa.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the OTUD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 192-220 from the human protein was used as the immunogen for the OTUD3 antibody.
Limitation:
This OTUD3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q5T2D3