Phospho-CREB1 Antibody (pS133)

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NSJ Bioreagents
Product Code: NSJ-F54407
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54407-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54407-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the phospho-CREB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of lysate from HeLa cells treated or untreated with TPA, using phospho-CREB antibody.
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Western blot testing of lysate from HeLa cells treated or untreated with TPA, using phospho-CREB antibody.
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Western blot testing of lysate from HeLa cells treated or untreated with Calyculin A, using phospho-CREB antibody.
4 / 5
IHC testing of FFPE human colon carcinoma tissue with phospho-CREB antibody antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
5 / 5
IHC testing of FFPE human hepatocarcinoma tissue with phospho-CREB antibody antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
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Western blot testing of lysate from HeLa cells treated or untreated with TPA, using phospho-CREB antibody.
Western blot testing of lysate from HeLa cells treated or untreated with TPA, using phospho-CREB antibody.
Western blot testing of lysate from HeLa cells treated or untreated with Calyculin A, using phospho-CREB antibody.
IHC testing of FFPE human colon carcinoma tissue with phospho-CREB antibody antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human hepatocarcinoma tissue with phospho-CREB antibody antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the phospho-CREB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
The amino acids surrounding phosphorylated serine 133 from the human protein were used as the immunogen for the phospho-CREB antibody.
Limitation:
This Phospho-CREB antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P16220