PIM2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54449
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54449-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54449-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PIM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) K562 and 2) SW620 cell lysate with PIM2 antibody. Predicted molecular weight ~34 kDa.
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Western blot testing of human HeLa cell lysate with PIM2 antibody. Predicted molecular weight ~34 kDa.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PIM2 antibody.
4 / 4
IHC testing of FFPE human cancer tissue with PIM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) K562 and 2) SW620 cell lysate with PIM2 antibody. Predicted molecular weight ~34 kDa.
Western blot testing of human HeLa cell lysate with PIM2 antibody. Predicted molecular weight ~34 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PIM2 antibody.
IHC testing of FFPE human cancer tissue with PIM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PIM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-2 is highly homologous to Pim-1 with similar oncogenic functions. Pim-2 overexpression promotes resistance to a host of apoptotic stimuli; its expression is negatively regulated by growth factor depletion. Increased levels of Pim-2 has also been observed in certain cancers.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 277-308 from the human protein was used as the immunogen for the PIM2 antibody.
Limitation:
This PIM2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
Q9P1W9