SIM1 Antibody / Single-minded homolog 1

NSJ Bioreagents
Product Code: NSJ-F54660
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54660-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54660-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Single-minded homolog 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human brain tissue with Single-minded homolog 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human kidney tissue with Single-minded homolog 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 293T cell lysate with Single-minded homolog 1 antibody. Predicted molecular weight ~86 kDa.
4 / 4
Western blot testing of human A549 cell lysate with Single-minded homolog 1 antibody. Predicted molecular weight ~86 kDa.

IHC testing of FFPE human brain tissue with Single-minded homolog 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with Single-minded homolog 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 293T cell lysate with Single-minded homolog 1 antibody. Predicted molecular weight ~86 kDa.
Western blot testing of human A549 cell lysate with Single-minded homolog 1 antibody. Predicted molecular weight ~86 kDa.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Single-minded homolog 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for the Single-minded homolog 1 antibody.
Limitation:
This Single-minded homolog 1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P81133