USP2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54760
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54760-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54760-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the USP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human kidney tissue with USP2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
IHC testing of FFPE human testis tissue with USP2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
Western blot testing of human HeLa cell lysate with USP2 antibody. Predicted molecular weight ~68 kDa.
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IHC testing of FFPE human kidney tissue with USP2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human testis tissue with USP2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HeLa cell lysate with USP2 antibody. Predicted molecular weight ~68 kDa.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the USP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
USP2, an ubiquitin-specific protease, is selectively up regulated in bone by the osteotropic agents PTH, PTHrP and PGE2 and possibly via the PKA/cAMP pathway. It is also thought to play a role in the recycling of ubiquitin by hydrolysis of branched poly-ubiquitin from linear poly-ubiquitin chains, production of free ubiquitin from linear poly-ubiquitin chains and from certain ribosomal ubiquitin fusion proteins.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 508-538 from the human protein was used as the immunogen for the USP2 antibody.
Limitation:
This USP2 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
O75604