ENTPD2 Antibody

NSJ Bioreagents
Product Code: NSJ-F54471
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54471-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54471-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ENTPD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC testing of FFPE human brain tissue with ENTPD2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of human 1) heart, 2) HEK293, 3) U-87 MG and 4) brain lysate with ENTPD2 antibody. Predicted molecular weight ~54 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human K562 cells with ENTPD2 antibody; Blue=isotype control, Green= ENTPD2 antibody.

IHC testing of FFPE human brain tissue with ENTPD2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) heart, 2) HEK293, 3) U-87 MG and 4) brain lysate with ENTPD2 antibody. Predicted molecular weight ~54 kDa.
Flow cytometry testing of fixed and permeabilized human K562 cells with ENTPD2 antibody; Blue=isotype control, Green= ENTPD2 antibody.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the ENTPD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ENTPD2 is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 86-113 from the human protein was used as the immunogen for the ENTPD2 antibody.
Limitation:
This ENTPD2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9Y5L3