PREX1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54365
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54365-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54365-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PREX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human brain tissue with PREX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human brain tissue with PREX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of human 1) MCF7, 2) SK-BR-3 and 3) ThP1 cell lysate with PREX1 antibody. Predicted molecular weight ~186 kDa.
4 / 5
Western blot testing of human NCI-H460 cell lysate with PREX1 antibody. Predicted molecular weight ~186 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with PREX1 antibody; Blue=isotype control, Green= PREX1 antibody.
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IHC testing of FFPE human brain tissue with PREX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with PREX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) MCF7, 2) SK-BR-3 and 3) ThP1 cell lysate with PREX1 antibody. Predicted molecular weight ~186 kDa.
Western blot testing of human NCI-H460 cell lysate with PREX1 antibody. Predicted molecular weight ~186 kDa.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with PREX1 antibody; Blue=isotype control, Green= PREX1 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the PREX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1134-1163 from the human protein was used as the immunogen for the PREX1 antibody.
Limitation:
This PREX1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q8TCU6