SCEL Antibody / Sciellin

NSJ Bioreagents
Product Code: NSJ-F54353
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54353-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54353-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Sciellin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human skin tissue with Sciellin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human esophagus tissue with Sciellin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human A431 cell lysate with Sciellin antibody. Predicted molecular weight ~77 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human A431 cells with Sciellin antibody; Blue=isotype control, Green= Sciellin antibody.

IHC testing of FFPE human skin tissue with Sciellin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human esophagus tissue with Sciellin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human A431 cell lysate with Sciellin antibody. Predicted molecular weight ~77 kDa.
Flow cytometry testing of fixed and permeabilized human A431 cells with Sciellin antibody; Blue=isotype control, Green= Sciellin antibody.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Sciellin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a precursor to the cornified envelope of terminally differentiated keratinocytes. This protein localizes to the periphery of cells and may function in the assembly or regulation of proteins in the cornified envelope. Transcript variants encoding different isoforms exist. A transcript variant utilizing an alternative polyA signal has been described in the literature, but its full-length nature has not been determined.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 260-289 from the human protein was used as the immunogen for the Sciellin antibody.
Limitation:
This Sciellin antibody is available for research use only.
Localization:
Cytoplasmic, plasma membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O95171