AKR1B1 Antibody / Aldose reductase

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NSJ Bioreagents
Product Code: NSJ-F54402
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54402-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54402-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the AKR1B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) A431 and 2) A549 cell lysate with AKR1B1 antibody. Predicted molecular weight ~36 kDa.
2 / 5
Western blot testing of human Jurkat cell lysate with AKR1B1 antibody. Predicted molecular weight ~36 kDa.
3 / 5
Western blot testing of 1) non-transfected and 2) transfected HEK293 cell lysate with AKR1B1 antibody.
4 / 5
Immunofluorescent staining of human HEK293 cells with AKR1B1 antibody (green) and DAPI nuclear stain (blue).
5 / 5
IHC testing of FFPE human colon carcinoma tissue with AKR1B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) A431 and 2) A549 cell lysate with AKR1B1 antibody. Predicted molecular weight ~36 kDa.
Western blot testing of human Jurkat cell lysate with AKR1B1 antibody. Predicted molecular weight ~36 kDa.
Western blot testing of 1) non-transfected and 2) transfected HEK293 cell lysate with AKR1B1 antibody.
Immunofluorescent staining of human HEK293 cells with AKR1B1 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human colon carcinoma tissue with AKR1B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the AKR1B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
AKR1B1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 102-135 from the human protein was used as the immunogen for the AKR1B1 antibody.
Limitation:
This AKR1B1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
P15121