ATG4B Antibody

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NSJ Bioreagents
Product Code: NSJ-F54715
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54715-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54715-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ATG4B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human HeLa cell lysate with ATG4B antibody. Expected molecular weight: 37-52 kDa (multiple isoforms).
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ATG4B antibody.
3 / 4
Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with ATG4B antibody (green) and Hoechst 33342 nuclear stain (blue).
4 / 4
IHC testing of FFPE human cancer tissue with ATG4B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HeLa cell lysate with ATG4B antibody. Expected molecular weight: 37-52 kDa (multiple isoforms).
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ATG4B antibody.
Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with ATG4B antibody (green) and Hoechst 33342 nuclear stain (blue).
IHC testing of FFPE human cancer tissue with ATG4B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the ATG4B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 358-390 from the human protein was used as the immunogen for the ATG4B antibody.
Limitation:
This ATG4B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q9Y4P1