ATG9A Antibody

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NSJ Bioreagents
Product Code: NSJ-F54719
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54719-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54719-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ATG9A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) A375 and 2) HepG2 cell lysate with ATG9A antibody. Predicted molecular weight ~94 kDa.
2 / 4
Western blot testing of human A2058 (left) and A375 (right) cell lysate with ATG9A antibody. Predicted molecular weight ~94 kDa.
3 / 4
Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with ATG9A antibody (green) and Hoechst 33342 nuclear stain (blue).
4 / 4
IHC testing of FFPE human breast cancer tissue with ATG9A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) A375 and 2) HepG2 cell lysate with ATG9A antibody. Predicted molecular weight ~94 kDa.
Western blot testing of human A2058 (left) and A375 (right) cell lysate with ATG9A antibody. Predicted molecular weight ~94 kDa.
Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with ATG9A antibody (green) and Hoechst 33342 nuclear stain (blue).
IHC testing of FFPE human breast cancer tissue with ATG9A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the ATG9A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 717-746 from the human protein was used as the immunogen for the ATG9A antibody.
Limitation:
This ATG9A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q7Z3C6